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SHORT COMMUNICATION
Year : 2017  |  Volume : 2  |  Issue : 1  |  Page : 44-46

Separation of autologous red blood cells from specimen of the transfused sickle cell disease patients using hypotonic saline


Lok Samarpan Regional Blood Bank and Research Center, Surat, Gujarat, India

Correspondence Address:
Sanmukh R Joshi
Lok Samarpan Regional Blood Bank and Research Center, Surat, Gujarat
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/GJTM.GJTM_1_17

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Background: Red blood cell (RBC) antigen typing in transfused sickle cell disease (SCD) patients is a difficult task due to contamination of the transfused red cells. Aim: This pilot study was aimed to standardize an easy approach to separate the autologous RBCs from the transfused patient for RBC antigen typing in SCD. Materials and Methods: The RBCs from transfused patients with SCD were separated by hypotonic saline and tested with various antisera by standard serological methods. Results: Ten antisera showing negative reaction with donors' RBCs reacted positively with the patients' autologous RBCs separated by treatment with hypotonic saline. Seven antisera reacted with the donors' RBCs were nonreactive with the patients' autologous RBCs after elimination of the donor's RBCs by treatment with hypotonic saline. Conclusion: The hypotonic saline can be used as method to separate the autologous RBCs from the mixture with the transfused RBC in patients with SCD.


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