|Year : 2018 | Volume
| Issue : 1 | Page : 79-80
Effect of traumatic hemorrhagic shock on the antioxidant enzyme systems of organism: An animal experimental study
Li Dapeng, Lv Chunlei, Wang Yongqing, Wang Fenjiao, Zheng Wenzhe
The 88th Hospital of PLA, Taian 271000, Shandong, China
|Date of Web Publication||5-Apr-2018|
The 88th Hospital of PLA, Taian 271000, Shandong
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Dapeng L, Chunlei L, Yongqing W, Fenjiao W, Wenzhe Z. Effect of traumatic hemorrhagic shock on the antioxidant enzyme systems of organism: An animal experimental study. Glob J Transfus Med 2018;3:79-80
|How to cite this URL:|
Dapeng L, Chunlei L, Yongqing W, Fenjiao W, Wenzhe Z. Effect of traumatic hemorrhagic shock on the antioxidant enzyme systems of organism: An animal experimental study. Glob J Transfus Med [serial online] 2018 [cited 2019 Mar 20];3:79-80. Available from: http://www.gjtmonline.com/text.asp?2018/3/1/79/229329
This Short communication is reproduced with permission from Journal of Clinical Transfusion and Lab Medicine
Traumatic hemorrhagic shock (THS) is a common acute critical illness in first-aid treatment. In this study, the THS model was established through a rapid blood loss to form a systolic pressure drop curve. The superoxide dismutase (SOD), hydrogen peroxide enzyme (CAT), and glutathione oxidation reductase (GSH-Px) were selected as monitoring index to evaluate the relationship between different blood loss and the antioxidant enzyme system during THS.
Eleven conventional New Zealand rabbits (Shandong Laboratory Center, SCXK (Lu) 2015-0001), body weighing 2–3 kg, and male or female unlimited were enrolled in this study. The rabbits were 24 h fasting and 2–3 h water deprivation, and the data including the basal systolic blood pressures were detected and recorded before the experiment. The right femoral artery of rabbit was selected for bloodletting, and the THS models were built through systolic blood pressure drawdown curve in the experiment. The systolic blood pressure was decreased accompany with the increasing of blood loss and then forming systolic blood pressure drawdown curve. When the systolic blood pressure decreased to1/1 (T1, Group A), 2/3 (T2, Group B), 1/2 (T3, Group C), and 1/3 (T4, Group D) of the basal systolic blood pressures, respectively, the blood samples were collected for >2 ml through the calibrated quantitative collection tube, and serum was separated rapidly by a conventional method (after centrifugation, the supernatant was stored at −80°C). Serum activity levels of antioxidant enzyme system, such as SOD, CAT, and GSH-Px, were detected with enzyme-linked immunosorbent assay (ELISA) at different spatial positions. The ELISA kit was from Nanjing Jiangchen Bioengineering Institute. Test value at different spatial positions and indicators of each group was statistically analyzed by F-test and least significant difference method.
The value from high to low of tested SOD was Group B, Group A, Group C, and Group D, whereas the value from high to low of tested CAT group and GSH-Px group was both A, B, C, and D [Table 1]. There were no significant differences among different time points in SOD group (P > 0.05) [Figure 1]. To CAT group, there were no significant differences between T1-T2 and T2-T3 subgroups (P > 0.05), whereas there were significant differences among other subgroups (P< 0.05). To GSH-Px group, there was no significant difference between T1 and T2 subgroups (P > 0.05), whereas significant differences were noted among the other subgroups (P< 0.05) [Figure 2].
|Table 1: The enzyme-linked immunosorbent assay results of tested superoxide dismutase, CAT, and GSH-Px|
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|Figure 1: The P value of time factor for superoxide dismutase, CAT, and GSH-Px group. The P value was gained by F-test. The result of superoxide dismutase group was high than 0.05, which showed no significant differences between different time points in superoxide dismutase group. While the results of CAT and GSH-Px group suggested that there were significant differences among different time points in the two groups. The data from the two groups need to be further analyzed|
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|Figure 2: The P value of subgroups in CAT and GSH-Px groups gained by least significant difference. To CAT group, there were no significant differences between T1 versus T2 and T2 versus T3 subgroups (P > 0.05), while there were significant differences among other subgroups (P < 0.05). To GSH-Px group, there was no significant difference between T1 versus T2 subgroups (P > 0.05), while significant differences were found among the rest subgroups (P < 0.05)|
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The results of our study revealed that there is a significant correlation between blood loss and activity of CAT and GSH-Px. The activity of SOD, CAT, and GSH-Px is decreased with the increasing of blood loss. The THS process has been significant effect on the antioxidant enzyme system. The increasing of blood loss can significantly reduce serum CAT and GSH-Px activity and also can reduce serum SOD activity although with no statistically significant differences. As a result, it can reduce the ability of free radical scavenging and the decrease of antioxidant capacity, promote lipid peroxidation, and lead to the damage of organism membrane.
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Journal of clinical Transfusion and Lab medicine.
[Figure 1], [Figure 2]