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ORIGINAL ARTICLE
Year : 2018  |  Volume : 3  |  Issue : 2  |  Page : 113-116

Use of two methods to elute anti-IgG and anti-(IgG + C3d) type autoantibody from sensitized erythrocytes


Dongguan City, Guangdong Province, China

Correspondence Address:
Dr. He Ziyi
Dongguan City, Guangdong Province
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/GJTM.GJTM_34_18

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Background: Autoimmune hemolytic anemia (AIHA) is an immunological disease caused by autoantibodies. Warm autoantibody mainly includes anti-IgG and anti-(IgG+C3d) type, which can cause direct antiglobulin test (DAT) positive. In order to correctly identify blood group and cross match, we compare the effect of using two elution methods to elute anti-IgG and anti-(IgG+C3d) type autoantibody sensitized erythrocyte. Methods: Twenty cases of anti-IgG type autoantibody and 20 cases of anti-(IgG+C3d) type autoantibody were eluted from sensitized erythrocytes using ZZAP (cysteine activated papain and dithiothreitol) and CP (chloroquine phosphate) reagents. Results: The effect of elution on anti-IgG type autoantibody sensitized erythrocytes is better than anti-(IgG+C3d) type with two elution methods (P < 0.05). The effect of absorption on anti-IgG and anti-(IgG+C3d) type autoantibody sensitized erythrocytes by ZZAP reagent is better than CP (P < 0.05), and the effect of absorption autoantibody after anti-IgG autoantibody sensitized erythrocytes with ZZAP is better than anti-(IgG+C3d) type (P < 0.05). Conclusion: Anti-(IgG+C3d) type autoantibody sensitized erythrocytes are all not eluted by ZZAP and CP, but ZZAP are better than CP on anti-IgG type autoantibody sensitized erythrocyte.


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