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| SPECIAL COMMUNICATION |
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| Year : 2018 | Volume
: 3
| Issue : 2 | Page : 142-143 |
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Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury
LW Li, ZQ Li
Department of Blood Transfusion, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China
| Date of Web Publication | 24-Oct-2018 |
Correspondence Address:
Dr. L W Li
Department of Blood Transfusion, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/GJTM.GJTM_42_18
How to cite this article:
Li L W, Li Z Q. Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury. Glob J Transfus Med 2018;3:142-3 |
How to cite this URL:
Li L W, Li Z Q. Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury. Glob J Transfus Med [serial online] 2018 [cited 2019 Feb 21];3:142-3. Available from: http://www.gjtmonline.com/text.asp?2018/3/2/142/243932 |
| Background | |  |
Transfusion-related acute lung injury (TRALI) is a serious blood transfusion complication characterized by the acute onset of noncardiogenic pulmonary edema following transfusion of blood products. Acute pancreatitis-associated lung injury (acute pancreatitis associated lung injury, APALI) is the most common and severe complication in patients with severe acute pancreatitis complicated with multiple system organ failure. Rapamycin is a new macrolide immunosuppressant. The recent report has shown that rapamycin could inhibit the inflammatory reaction induced by NF-B activation and neutrophil, but till now, the effect of rapamycin in acute lung injury caused by blood transfusion and acute pancreatitis is unknown. The amplification curves of real-time fluorescent quantitative PCR showed obvious “S” type [Figure 1]a, [Figure 1]b,[Figure 1]c. The melting curve showed no impurity peak and no abnormal broadening of the main peak occurred [Figure 2]a, [Figure 2]b, [Figure 2]c. | Figure 1: Gene amplification curves: (a) Reference gene β-actin, (b) target gene NF-κB p65 and (c) target gene STAT-1
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 | Figure 2: Melting curves: (a) Reference gene β-actin, (b) target gene NF-κB p65 and (c) target gene STAT-1
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Objective
To observe the effect of rapamycin on gene expression of nuclear factor-κB (NF-κB) p65 and STAT-1 in Sprague–Dawley rats with acute lung injury.
| Methods | | |
The animal models of TRALI and APALI were prepared using Sprague–Dawley rats. Histopathological examination of lung tissues validated acute lung injury. Gene expression in NF-κB p65 and STAT-1 was detected by real-time fluorescent qualification RT-PCR on the control of beta-actin (housekeeping gene).
| Results | | |
The gene expression of NF-κB p65 and STAT-1 in TRALI and APALI group was downregulated to a different extent (P < 0.05), and APALI group decreased more than TRALI group (P < 0.01). The gene expression of NF-κB p65 and STAT-1 in rapamycin intervention TRALI group decreased more than TRALI group (P < 0.01), while rapamycin intervention APALI group did not decrease significantly compared to APALI group (P > 0.05).
| Conclusions | | |
In the present study, we first established TRALI and acute pancreatitis-associated lung injury SD rat model and then observed the effect of rapamycin on gene expression of NF-κB p65 and STAT-1 in Sprague–Dawley Rats with acute lung injury induced by blood transfusion. Further aggravating the lung injury of TRALI, rapamycin had limited effects on APALI. For detailed article log on to: Journal of Clinical Transfusion and Lab Medicine http://www.lcsxyjy.com/CN/10.3969/j.issn.1671-2587.2016.03.002
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
[Figure 1], [Figure 2]
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