Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online:94
  • Home
  • Print this page
  • Email this page


 
 Table of Contents  
SPECIAL COMMUNICATION
Year : 2018  |  Volume : 3  |  Issue : 2  |  Page : 142-143

Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury


Department of Blood Transfusion, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China

Date of Web Publication24-Oct-2018

Correspondence Address:
Dr. L W Li
Department of Blood Transfusion, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/GJTM.GJTM_42_18

Rights and Permissions

How to cite this article:
Li L W, Li Z Q. Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury. Glob J Transfus Med 2018;3:142-3

How to cite this URL:
Li L W, Li Z Q. Effect of rapamycin on gene expression of nuclear factor-κB p65 and STAT-1 in sprague–dawley rats with acute lung injury. Glob J Transfus Med [serial online] 2018 [cited 2018 Dec 16];3:142-3. Available from: http://www.gjtmonline.com/text.asp?2018/3/2/142/243932




  Background Top


Transfusion-related acute lung injury (TRALI) is a serious blood transfusion complication characterized by the acute onset of noncardiogenic pulmonary edema following transfusion of blood products. Acute pancreatitis-associated lung injury (acute pancreatitis associated lung injury, APALI) is the most common and severe complication in patients with severe acute pancreatitis complicated with multiple system organ failure. Rapamycin is a new macrolide immunosuppressant. The recent report has shown that rapamycin could inhibit the inflammatory reaction induced by NF-B activation and neutrophil, but till now, the effect of rapamycin in acute lung injury caused by blood transfusion and acute pancreatitis is unknown. The amplification curves of real-time fluorescent quantitative PCR showed obvious “S” type [Figure 1]a, [Figure 1]b,[Figure 1]c. The melting curve showed no impurity peak and no abnormal broadening of the main peak occurred [Figure 2]a, [Figure 2]b, [Figure 2]c.
Figure 1: Gene amplification curves: (a) Reference gene β-actin, (b) target gene NF-κB p65 and (c) target gene STAT-1

Click here to view
Figure 2: Melting curves: (a) Reference gene β-actin, (b) target gene NF-κB p65 and (c) target gene STAT-1

Click here to view


Objective

To observe the effect of rapamycin on gene expression of nuclear factor-κB (NF-κB) p65 and STAT-1 in Sprague–Dawley rats with acute lung injury.


  Methods Top


The animal models of TRALI and APALI were prepared using Sprague–Dawley rats. Histopathological examination of lung tissues validated acute lung injury. Gene expression in NF-κB p65 and STAT-1 was detected by real-time fluorescent qualification RT-PCR on the control of beta-actin (housekeeping gene).


  Results Top


The gene expression of NF-κB p65 and STAT-1 in TRALI and APALI group was downregulated to a different extent (P < 0.05), and APALI group decreased more than TRALI group (P < 0.01). The gene expression of NF-κB p65 and STAT-1 in rapamycin intervention TRALI group decreased more than TRALI group (P < 0.01), while rapamycin intervention APALI group did not decrease significantly compared to APALI group (P > 0.05).


  Conclusions Top


In the present study, we first established TRALI and acute pancreatitis-associated lung injury SD rat model and then observed the effect of rapamycin on gene expression of NF-κB p65 and STAT-1 in Sprague–Dawley Rats with acute lung injury induced by blood transfusion. Further aggravating the lung injury of TRALI, rapamycin had limited effects on APALI. For detailed article log on to: Journal of Clinical Transfusion and Lab Medicine http://www.lcsxyjy.com/CN/10.3969/j.issn.1671-2587.2016.03.002

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.




    Figures

  [Figure 1], [Figure 2]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Background
Methods
Results
Conclusions
Article Figures

 Article Access Statistics
    Viewed83    
    Printed3    
    Emailed0    
    PDF Downloaded7    
    Comments [Add]    

Recommend this journal


[TAG2]
[TAG3]
[TAG4]