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SPECIAL COMMUNICATION
Year : 2020  |  Volume : 5  |  Issue : 1  |  Page : 113-114

Expression of CD200/CD200R1 from peripheral blood mononuclear cells of primary immune thrombocytopenia and its clinical significance


Department of Transfusion, Shanghai Changhai Hospital, Second Military Medical University, Shanghai, China

Date of Submission01-Apr-2020
Date of Decision01-Apr-2020
Date of Acceptance01-Apr-2020
Date of Web Publication17-Apr-2020

Correspondence Address:
Qian Bao-Hua
Department of Transfusion, Shanghai Changhai Hospital, Second Military Medical University, Shanghai
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/GJTM.GJTM_27_20

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How to cite this article:
Yan Z, Xin Y, Bao-Hua Q. Expression of CD200/CD200R1 from peripheral blood mononuclear cells of primary immune thrombocytopenia and its clinical significance. Glob J Transfus Med 2020;5:113-4

How to cite this URL:
Yan Z, Xin Y, Bao-Hua Q. Expression of CD200/CD200R1 from peripheral blood mononuclear cells of primary immune thrombocytopenia and its clinical significance. Glob J Transfus Med [serial online] 2020 [cited 2020 Aug 9];5:113-4. Available from: http://www.gjtmonline.com/text.asp?2020/5/1/113/282734




  Introduction Top


Primary immune thrombocytopenia (ITP) is a common autoimmune hemorrhagic disease in the clinic and is characterized by isolated thrombocytopenia (peripheral blood platelet count <100 × 109/L) in the absence of other causes or disorders that may be associated with thrombocytopenia. The main clinical problem of primary ITP is an increased risk of bleeding, although bleeding symptoms may not always be present. The pathological feature is that patients produce antibodies against their platelets, leading to increased platelet destruction and an impediment to production. The common clinical manifestations are petechial of the skin; severe cases may have uncontrollable internal organs and skull bleeding, which seriously endangers the life and health of patients. Glucocorticoids are still the first-line treatment for newly diagnosed ITP patients. Similar to most autoimmune diseases, the imbalance of autoimmune homeostasis is a key factor leading to the disease. Many immune cells are involved in this process, such as autoreactive T cells, regulatory T cells, B cells, and monocyte. At present, most studies believe that T cell dysfunction plays a decisive role in the pathogenesis of ITP, specifically the excessive activation of Th1/17 subtype cells and secretion of tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and interleukin-17 (IL-17), leading to patients with autoimmune hyperactivity. CD200 is a type I membrane glycoprotein that is widely expressed in a variety of cells and tissues. Through its receptor CD200R1, it transmits response signals that affect a variety of physiological systems. CD200 is involved in the pathogenesis of many diseases, including tumors, allergic diseases, and autoimmune diseases. CD200/CD200R1 molecule can suppress immune system response and maintain peripheral immune tolerance. However, it is unclear whether the signal pathway molecule is abnormally expressed in patients with ITP and whether it is related to the autoimmune disorder of ITP.
Figure 1: CD200/CD200R1 expression difference between immune thrombocytopenia patients and healthy controls

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Objective

To investigate the expression of CD200 and its receptor CD200R1 in peripheral blood PBMCs from ITP patients, analyze its relationship with the Th1/17 cell hyperthyroidism in ITP patients, and provide new experimental evidence for further understanding of the pathogenesis of ITP. Provide potential targets for possible immunomodulatory therapies for ITP.{Tbale 1}{Tbale 2}
Figure 2: Tumor necrosis factor-α, interferon-γ, and interleukin-17 protein expression in plasma of immune thrombocytopenia patients *P < 0.05

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  Methods Top


The study cohort included 19 patients with ITP and 19 healthy controls (HCs). We first compared the mRNA relative expression of CD200/CD200R1 and the protein levels of TNF-α, IFN-γ, and IL-17 in plasma between HCs and patients with ITP. We further analyzed the correlation between CD200/CD200R1 expression and platelet count. The changes of CD200/CD200R1 expression after glucocorticoids treatment were also evaluated by RT-PCR. Statistical significance was determined using group t-test or paired t-test, and correlation analysis using Spearman's rank correlation method. All P values were two-sided, and values <0.05 were considered statistically significant. Statistical analysis was determined using GraphPad Prism software 23.0.


  Results Top


The CD200/CD200R1 expression in patients with ITP was lower than that in HCs (P < 0.05), and TNF-α, IFN-γ, and IL-17 protein expressions showed the opposite trend. The level of TNF-α and IFN-γ showed a negative correlation with CD200/CD200R1, and platelet count showed a positive correlation with CD200/CD200R1 in ITP patients. Moreover, the mRNA expression of CD200/CD200R1 showed an increasing trend in ITP patients sensitive to dexamethasone.


  Conclusion Top


The expression and function of CD200/CD200R1 played an important role in the pathogenesis of ITP patients, which may regulate the disease process of patients by increasing the inflammatory cytokines. Research in this area might provide noteworthy data to better understand pathogenesis related to ITP.

For detailed article log on to: Journal of Clinical Transfusion and Lab Medicine Available from: http://www.lcsxyjy.com/CN/10.3969/j.issn. 1671-2587.2020.01.00 2.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.




    Figures

  [Figure 1], [Figure 2]
 
 
    Tables

  [Table 1], [Table 2]



 

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