Global Journal of Transfusion Medicine

: 2020  |  Volume : 5  |  Issue : 1  |  Page : 115--116

Fresh frozen plasma: A new perspective

Subhashish Das 
 Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka, India

Correspondence Address:
Subhashish Das
Department of Pathology, Sri Devaraj Urs Medical College, Kolar, Karnataka

How to cite this article:
Das S. Fresh frozen plasma: A new perspective.Glob J Transfus Med 2020;5:115-116

How to cite this URL:
Das S. Fresh frozen plasma: A new perspective. Glob J Transfus Med [serial online] 2020 [cited 2020 Nov 24 ];5:115-116
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With reference to the article “Loganathan R, Kulkarni RG, Kar R, Abhishekh B, Basu D. Assessment and association of coagulation factors (FVIII and fibrinogen) with the mode of collection and storage of fresh frozen plasma (FFP). Glob J Transfus Med 2019;4:204-7,” I have the following opinion to be shared.

Preanalytical conditions in laboratory assessment of coagulation systems and hemostasis are very important.[1] Preanalytical variables which can effect coagulating test and factor analysis results are specimen collection, hematocrit, transportation, filling status of the sampling tube, centrifuge, anticoagulant type and concentration, as well as storage and assay method.[2]

Many studied have suggested acceptable storage temperature for routine coagulation test.[3] Although the variable influences of storage, temperature and time on Factor IX and Factor VIII in FFP have been reported.[4]

To diagnose and treat hemophilia and to monitor oral anticoagulation therapy, thrombotic disease, and chronic liver disease, timely and accurate coagulation tests and factor detection in fresh plasma samples are very important.[5]

To analyze the effect of plasma storage and study on factor VIII and fibrinogen, several studies have been done. Kemkes Matthes et al. have reported that the acceptable time interval for the storage of APTT, activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen, and D dimer after storage for 8 hours at room temperature which can be extended to 24 hours for PT, TT, and D dimer determination. Van Geest Daalderop et al. have reported that the acceptable time interval is 6 hours at 4–6°C, for PT/INR determination. Rao LV et al.[6] have reported that whole blood and plasma samples can be tested for APTT for up to 12 h and for PT for up to 24 h, when transported either at room temperature or at 4°C.[7]

In clinical practice, coagulation tests and factor measurements have been widely applied. Therefore, it is necessary to evaluate the effects of time from collection and temperature on the outcome of these results. The plasma samples tested could be safely stored for up to 24 h both at 4°C and 25°C for fibrinogen, PT/INR, and TT determination; those tested for APTT measurement could be safely stored for 12 h at 4°C and 8 h at 25°C; those tested for FIX: C measurement could be safely stored for 4 h at 4°C and 25°C; and measurement of FVIII-C should be done immediately that is within 2 h after collection in our laboratory. Thus, clinical samples should only be stored for these time frames before testing.[8]


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